Huetz F, Carlsson L, Tornberg U C, Holmberg D
Unit for Applied Cell and Molecular Biology, University of Umeå, Sweden.
EMBO J. 1993 May;12(5):1819-26. doi: 10.1002/j.1460-2075.1993.tb05830.x.
We here analyse the repertoire of VH7183 rearrangements isolated from different stages of B cell differentiation in adult mice. The nucleotide sequence analyses of VH7183-D-JH rearrangements derived from large pre-B cells (B220+, mu-), small pre-B cells (B220+, mu-) and mature B cells (B220+, mu+) isolated from adult bone marrow revealed a sequential accumulation, among functional rearrangements, of D segments of the FL16 family and a depletion of D segments using the second and the third reading frame (RF). One member (VH7183.1) of the VH7183 gene family was utilized in 60-80% of the rearrangements of all populations analysed. In neonates the majority of the rearrangements utilizing this gene was found to be functional. In contrast, > 96% of the VH7183 rearrangements isolated from adult spleen were non-functional. These data provide evidence for cellular selection of VH regions acting at different points of the B cell differentiation pathway and at the transition of B cells from the bone marrow to the periphery.
我们在此分析从成年小鼠B细胞分化不同阶段分离出的VH7183重排库。对从成年骨髓中分离出的大前B细胞（B220 +，μ-）、小前B细胞（B220 +，μ-）和成熟B细胞（B220 +，μ+）的VH7183 - D - JH重排进行核苷酸序列分析，结果显示，在功能性重排中，FL16家族的D片段呈顺序积累，而使用第二和第三阅读框（RF）的D片段则减少。VH7183基因家族的一个成员（VH7183.1）在所有分析群体的60 - 80%的重排中被使用。在新生儿中，利用该基因的大多数重排被发现是功能性的。相比之下，从成年脾脏中分离出的VH7183重排中有超过96%是非功能性的。这些数据为B细胞分化途径不同点以及B细胞从骨髓向外周转变过程中VH区域的细胞选择提供了证据。
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