Morasso M I, Mahon K A, Sargent T D
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892-2790, USA.
Proc Natl Acad Sci U S A. 1995 Apr 25;92(9):3968-72. doi: 10.1073/pnas.92.9.3968.
In this paper, we show the conserved regulation of the homeodomain gene Distal-less-3 (Dlx-3) by analyzing the expression of a promoter from the Xenopus ortholog, Xdll-2, in transgenic mice. A 470-bp frog regulatory sequence confers appropriate expression on a lacZ reporter gene in the ectodermal component of structures derived from epithelial-mesenchymal interactions. Remarkably, this includes structures absent in Xenopus, such as the hair follicle and mammary gland, suggesting that conserved regulatory elements can be used to control the formation of structures peculiar to individual species. In addition, expression of Dlx-3 in developing limbs is highest at the most distal portion. This pattern is duplicated by the Xenopus promoter, indicating that this DNA may include sequences responsive to conserved proximodistal patterning signals in the vertebrate limb.
在本文中，我们通过分析非洲爪蟾直系同源基因Xdll-2的启动子在转基因小鼠中的表达，展示了同源异型结构域基因远端缺失-3（Dlx-3）的保守调控。一段470bp的青蛙调控序列赋予了源自上皮-间充质相互作用的结构的外胚层成分中的lacZ报告基因以适当的表达。值得注意的是，这包括非洲爪蟾中不存在的结构，如毛囊和乳腺，这表明保守的调控元件可用于控制个别物种特有的结构的形成。此外，Dlx-3在发育中的肢体中最远端部分的表达最高。非洲爪蟾启动子重复了这种模式，表明该DNA可能包含对脊椎动物肢体中保守的近远轴模式信号有反应的序列。
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