梨形四膜虫DNA聚合酶活性对阿糖胞苷三磷酸和N-乙基马来酰亚胺的敏感性。
Sensitivity of the DNA polymerase activities of Tetrahymena pyriformis to cytosine arabinoside triphosphate and N-ethyl-maleiimide.
作者信息
Benhura M A
机构信息
Department of Biochemistry, University of Zimbabwe.
出版信息
Pharmazie. 1995 Mar;50(3):213-5.
PMID:7732054
Abstract
Two DNA polymerase activities from Tetrahymena pyriformis were studied. The cytoplasmic polymerase was inhibited by cytosine arabinoside triphosphate (ara-CTP) and N-ethylmaleiimide (NEM) whereas the nuclear enzyme was not inhibited by araCTP and was inhibited by NEM to a lesser extent. The two enzymes could use CsCl, NaCl, and LiCl instead of KCl to varying degrees in the polymerase reaction. The two activities showed optimum activity at different concentrations of NaCl and KCl.
摘要
对来自梨形四膜虫的两种DNA聚合酶活性进行了研究。胞质聚合酶受到三磷酸阿糖胞苷（ara-CTP）和N-乙基马来酰亚胺（NEM）的抑制，而核酶不受araCTP抑制，且受NEM抑制的程度较小。在聚合酶反应中，这两种酶可以不同程度地使用CsCl、NaCl和LiCl来替代KCl。这两种活性在不同浓度的NaCl和KCl下表现出最佳活性。
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