Concannon M J, Boschert M T, Fitzpatrick L, Croll G H, Puckett C L
Division of Plastic Surgery, University of Missouri, Columbia, USA.
Plast Reconstr Surg. 1995 May;95(6):1085-91. doi: 10.1097/00006534-199505000-00021.
Demineralized bone powder has been shown previously to successfully induce bone formation. The purpose of this study was to use demineralized bone powder in an onlay graft situation using implantable molds. Demineralized bone powder was placed within a mold and rigidly attached to the frontal bones of 30 New Zealand White rabbits by screw fixation. Periosteum was not closed over the mold. A control group had the mold placed without demineralized bone powder used. After 12 weeks of growth, the animals were sacrificed and the frontal bone was analyzed for bone growth using standardized histology and fluorescent microscopy. One hundred percent of the animals (30 of 30) that received demineralized bone powder underwent bone growth in the precise shape of the mold (p < 0.001). There was no new bone development in the control group (n = 8). Placement of larger molds duplicated these results (n = 20) with a 650 percent increase in frontal bone thickness within 12 weeks after placement of demineralized bone powder. This model mimics onlay bone grafting, and perhaps this technique may replace onlay grafting or alloplastic implantation in certain circumstances, avoiding the extra morbidity and donor-site defects associated with bone grafting.
脱矿骨粉此前已被证明能成功诱导骨形成。本研究的目的是在使用可植入模具的覆盖植骨情况下使用脱矿骨粉。将脱矿骨粉置于模具内，通过螺钉固定牢固地附着于30只新西兰白兔的额骨上。模具上方的骨膜未闭合。对照组放置未使用脱矿骨粉的模具。生长12周后，处死动物，使用标准化组织学和荧光显微镜分析额骨的骨生长情况。接受脱矿骨粉的动物（30只中的30只）100%在模具的精确形状内实现了骨生长（p<0.001）。对照组（n=8）没有新骨形成。放置更大的模具（n=20）也得到了相同的结果，在放置脱矿骨粉后12周内额骨厚度增加了650%。该模型模拟了覆盖骨移植，也许在某些情况下，这种技术可以替代覆盖移植或异体植入，避免与骨移植相关的额外发病率和供区缺损。
