Roa I, Araya J C, Shiraishi T, Yatani R, Wistuba I, Villaseca M, de Aretxabala X
Unidad de Anatomía Patológica y Citopatología, Facultad de Medicina, Universidad de La Frontera, Hospital Regional de Temuco, Chile.
Rev Med Chil. 1994 Jul;122(7):754-9.
The expression of c-myc and p-ras-21 oncogenes was studied using an immunohistochemical method with monoclonal antibodies in 126 samples of gallbladder carcinoma (103 primary tumors and 23 metastatic lesions). Twenty five gallbladder samples without tumor evidence were used as controls. C-myc oncoprotein was positive in one control sample and p-ras-21 oncoprotein was negative in all. Among primary carcinomas c-myc was positive in 9 (9%) and p-ras-21 in 4 (4%); among metastatic carcinomas c-myc was positive in 6 (26%, p = 0.03 vs primary carcinoma) and p-ras-21 in 11 (48%, p < 0.001 vs primary carcinoma). There was a non significant association between c-myc and p-ras-21 expression and degree of histological differentiation and level of tumoral infiltration. It is concluded that c-myc and p-ras-21 oncoprotein expression is observed in less than 10% of primary gallbladder carcinomas and that this expression is significantly higher in metastatic carcinomas. This may reflect an activation or overexpression of these oncogenes in metastatic cells.
采用单克隆抗体免疫组化方法，对126例胆囊癌标本（103例原发性肿瘤和23例转移灶）进行c-myc和p-ras-21癌基因表达研究。选取25例无肿瘤证据的胆囊标本作为对照。c-myc癌蛋白在1例对照标本中呈阳性，p-ras-21癌蛋白在所有对照标本中均呈阴性。在原发性癌中，c-myc阳性9例（9%），p-ras-21阳性4例（4%）；在转移性癌中，c-myc阳性6例（26%，与原发性癌相比p = 0.03），p-ras-21阳性11例（48%，与原发性癌相比p < 0.001）。c-myc和p-ras-21表达与组织学分化程度及肿瘤浸润水平之间无显著相关性。研究得出结论，原发性胆囊癌中c-myc和p-ras-21癌蛋白表达率低于10%，而在转移性癌中该表达明显更高。这可能反映了这些癌基因在转移细胞中的激活或过度表达。
