Pelczar P, Fiett J, Bartnik E
Department of Genetics, University of Warsaw, Poland.
Acta Biochim Pol. 1994;41(4):449-52.
We have estimated the number of 5S rRNA genes in Aspergillus nidulans using two-dimensional agarose gel electrophoresis and hybridization to appropriate probes, representing the 5'-halves, the 3'-halves of the 5S rRNA sequence and a sequence found at the 3'-end of all known A. nidulans pseudogenes (block C). We have found 23 5S rRNA genes, 15 pseudogenes consisting of the 5'-half of the 5S rRNA sequence (of which 3 are flanked by block C) and 12 copies of block C which do not seem to be in the vicinity of 5S rRNA sequences. This number of genes is much lower than our earlier estimates, and makes our previously analyzed sample of 9 sequenced genes and 3 pseudogenes much more representative.
我们使用二维琼脂糖凝胶电泳以及与合适探针杂交的方法，估算了构巢曲霉中5S rRNA基因的数量。这些探针分别代表5S rRNA序列的5'端一半、3'端一半以及在所有已知构巢曲霉假基因（C区）3'端发现的一个序列。我们发现了23个5S rRNA基因、15个由5S rRNA序列5'端一半组成的假基因（其中3个两侧有C区）以及12个似乎不在5S rRNA序列附近的C区拷贝。这个基因数量比我们之前的估计要低得多，使得我们之前分析的9个已测序基因和3个假基因的样本更具代表性。
