Tsyganenko A Ia, Balakliets N I
Antibiotiki. 1975;20(12):1107-11.
Biochemical activity of the guinea pig leucocytes phagocyting live antibiotic sensitive and resistant bacteria was studied in vitro. The results of the study showed that the leucocytes which had absorbed live Staph. aureus and E. coli sensitive and resistant to be benzylpenicillin and levomycetin respectively differed in increased contents of nucleic acids. The increase was due to higher contents of RNA. The activity of dehydrogenases, acid and alkaline phosphatases was markedly inhibited. The use of oxygen and lipase activity did not differ from the control in the experiments with phagocytosis of both Staph. aureus and E. coli. The activity of peroxidase and catalase in the experiments with phagocytosis of E. coli did not differ from the control, while in the experiments with phagocytosis of Staph. aureus it increased. The data testified to the fact that the enzymatic activity of the phagocytes changed when they absorbed the objects of phagocytosis, the changes in the biochemical activity being dependent on the nature of the antigen and its state.
在体外研究了豚鼠白细胞吞噬对抗生素敏感和耐药的活细菌的生化活性。研究结果表明，分别吞噬对苄青霉素和氯霉素敏感及耐药的活金黄色葡萄球菌和大肠杆菌的白细胞，其核酸含量增加。这种增加是由于RNA含量较高。脱氢酶、酸性和碱性磷酸酶的活性受到明显抑制。在吞噬金黄色葡萄球菌和大肠杆菌的实验中，氧气的利用和脂肪酶活性与对照无差异。在吞噬大肠杆菌的实验中，过氧化物酶和过氧化氢酶的活性与对照无差异，而在吞噬金黄色葡萄球菌的实验中，其活性增加。这些数据证明，吞噬细胞在吞噬吞噬对象时其酶活性发生变化，生化活性的变化取决于抗原的性质及其状态。
