Shimaoka M, Iida T, Ohara A, Taenaka N, Mashimo T, Honda T, Yoshiya I
Research Institute for Microbial Diseases, Osaka University, Japan.
Biochem Biophys Res Commun. 1995 Apr 17;209(2):519-26. doi: 10.1006/bbrc.1995.1532.
The ability of exogenous nitric oxide (NO) to induce apoptosis in macrophages was analyzed using NOC, a NO-releasing compound, as a source of NO. Exogenous NO was shown to induce apoptosis in a dose dependent manner. Quantitative analysis revealed that the amount of NO required to induce apoptosis in more than half of macrophages exposed was 100 times larger than that for endogenous NO-induced apoptosis. NOC proved to be a convenient and useful tool for investigation of apoptosis related to NO.
使用一氧化氮释放化合物NOC作为一氧化氮（NO）的来源，分析外源性NO诱导巨噬细胞凋亡的能力。结果表明，外源性NO以剂量依赖性方式诱导凋亡。定量分析显示，在半数以上暴露的巨噬细胞中诱导凋亡所需的NO量比内源性NO诱导凋亡所需的量高100倍。NOC被证明是研究与NO相关凋亡的一种方便且有用的工具。
