Watanabe M, Umenai T, Ohori H, Ishida N
Br J Exp Pathol. 1976 Apr;57(2):211-6.
Growth conditions fof human oval cells (immature hepatocytes),evidence of hepatitis B (HB) antigen synthesis in oval cells as revealed by immunofluorescent staining and successful passage of such an agent in the culture fluid up to the 4th passage are described. The results have been proved to be readily reproducible with different inocula. The oval cells used in these experiments were defined as small round cells, with scant cytoplasm, vesicular nuclei and small nucleoli, vitally stained with indocyanine green and synthesizing alpha-foetoprotein but no albumin.
描述了人类卵圆细胞（未成熟肝细胞）的生长条件，通过免疫荧光染色揭示卵圆细胞中乙肝（HB）抗原合成的证据，以及这种因子在培养液中成功传代至第4代的情况。结果已被证明用不同接种物很容易重复。这些实验中使用的卵圆细胞被定义为小圆形细胞，细胞质稀少，核呈泡状，核仁小，用吲哚菁绿进行活体染色，能合成甲胎蛋白但不合成白蛋白。
