Caimi G, Lo Presti R, Montana M, Contorno A, Canino B, Catania A, Sarno A, Cerasola G
Istituto di Clinica Medica e Malattie Cardiovascolari, Università degli Studi di Palermo, Italy.
Am J Hypertens. 1995 Jan;8(1):82-6. doi: 10.1016/0895-7061(94)00183-C.
In a group of subjects with essential hypertension platelets were studied in resting conditions: platelet membrane fluidity was measured with the fluorescent probe 1.4-(trimethylamino)-phenyl-4-phenylhexatriene (TMA-DPH), platelet membrane cholesterol/phospholipid ratio was evaluated separating the membrane lipids with column chromatography, and platelet membrane individual phospholipids were determined using two-dimensional thin-layer chromatography. From the obtained results, it is evident that platelet membrane fluidity does not differentiate normals from hypertensives; platelet membrane cholesterol/phospholipid ratio is increased in hypertensives, while of the platelet membrane individual phospholipids, only the phosphatidylcholine is increased. In normals and hypertensives, no relation is evident between platelet membrane fluidity, platelet membrane lipid pattern, and systolic and diastolic blood pressure values.
在一组原发性高血压患者中，对静息状态下的血小板进行了研究：用荧光探针1,4-（三甲基氨基）-苯基-4-苯基己三烯（TMA-DPH）测量血小板膜流动性，通过柱色谱法分离膜脂来评估血小板膜胆固醇/磷脂比率，并使用二维薄层色谱法测定血小板膜中的单个磷脂。从所得结果可以明显看出，血小板膜流动性无法区分正常人和高血压患者；高血压患者的血小板膜胆固醇/磷脂比率升高，而在血小板膜的单个磷脂中，只有磷脂酰胆碱增加。在正常人和高血压患者中，血小板膜流动性、血小板膜脂质模式与收缩压和舒张压值之间均无明显关联。
