Díaz-Nido J, Mizuno K, Nawa H, Marshak D R
Beckman Neuroscience Center, Cold Spring Harbor Laboratory, NY 11724.
Cell Mol Biol Res. 1994;40(5-6):581-5.
Protein kinase CK2 is very abundant in rat brain when compared with other rat tissues. The enzyme is an oligomeric protein with the structures alpha 2 beta 2, alpha alpha'beta 2 and alpha'2 beta 2. The alpha and alpha' subunits are catalytic and have a high degree of homology, whereas the beta subunit seems regulatory. Using specific antibodies to synthetic peptide antigens that discriminate among the alpha, alpha', and beta subunits of CK2, we have observed a significant increase in the amount of alpha' subunit during the late postnatal neocortical maturation period. The increased alpha' expression occurs at a parallel time to synaptogenesis. As for its distribution, the alpha' subunit of CK2 is much more abundant in neurons (particularly in large size neurons) than it is in glia. These results are consistent with a hypothetical role for CK2 isoforms containing alpha' subunits in the regulation of specific functions in fully differentiated neurons.
与大鼠的其他组织相比，蛋白激酶CK2在大鼠脑中含量极为丰富。该酶是一种寡聚蛋白，具有α2β2、αα'β2和α'2β2结构。α和α'亚基具有催化作用且同源性很高，而β亚基似乎起调节作用。利用针对能区分CK2的α、α'和β亚基的合成肽抗原的特异性抗体，我们观察到在出生后新皮质成熟后期α'亚基的量显著增加。α'表达增加与突触形成同时发生。至于其分布，CK2的α'亚基在神经元（特别是大型神经元）中比在神经胶质细胞中丰富得多。这些结果与含有α'亚基的CK2同工型在完全分化的神经元中调节特定功能的假设作用一致。
