Binns M M, Holmes N G, Holliman A, Scott A M
Animal Health Trust, Kennett, Newmarket, Suffolk, UK.
Br Vet J. 1995 Jan-Feb;151(1):9-15. doi: 10.1016/s0007-1935(05)80057-0.
Six new horse microsatellite loci were identified by sequencing M13 clones containing horse genomic inserts which gave positive signals when probed with a CA/GT repeat probe. Oligonucleotide primer pairs were synthesized for these loci and for two previously described horse microsatellites, HTG4 and HTG6. Polymerase chain reaction assays were then carried out on a panel of 20 different unrelated Thoroughbred horse DNAs. DNAs from eight cases of double covering which could not be solved by conventional blood typing were also examined. Several of the loci amplified were found to be polymorphic and, using a limited subset of primers, a clear exclusion could be established for one of the stallions in five of the cases. DNA typing is therefore a useful adjunct to blood typing in the horse and indeed, in the future will probably replace it.
通过对含有马基因组插入片段的M13克隆进行测序，鉴定出六个新的马微卫星基因座，当用CA/GT重复探针进行探测时，这些克隆会产生阳性信号。为这些基因座以及两个先前描述的马微卫星HTG4和HTG6合成了寡核苷酸引物对。然后对一组20个不同的无亲缘关系的纯种马DNA进行聚合酶链反应分析。还检测了八例常规血型鉴定无法解决的双覆盖病例的DNA。发现扩增的几个基因座具有多态性，并且使用有限的引物子集，在五个病例中的一个种马中可以明确排除。因此，DNA分型是马血型鉴定的有用辅助手段，实际上，在未来可能会取代它。
