Sunairi M, Watanabe K, Suzuki T, Tanaka N, Kuwayama H, Nakajima M
Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Kanagawa, Japan.
Eur J Gastroenterol Hepatol. 1994 Dec;6 Suppl 1:S121-4.
To investigate the mechanism of action of a series of potential combination therapies for use against Helicobacter pylori.
The effects of certain anti-ulcer agents on the antimicrobial activity of antibiotics effective against H. pylori were determined in vitro.
H. pylori was cultured on Skirrow's agar. Amoxycillin, clarithromycin, erythromycin and tetracycline were used. The anti-ulcer agents studied comprised aluminum chloride, sofalcone [2'-carboxymethyl 4,4'-bis(3-methyl-2-butenyloxy)chalcone] and zinc chloride. Urease activity was measured by the urease-indophenol method. The minimum inhibitory concentration was determined by a plating method, with H. pylori streaked on plates containing various concentrations of the antibiotics plus sublethal doses of the anti-ulcer agents.
This in vitro study showed that sofalcone had a direct antibacterial effect and, in addition, inhibited the adhesive property of H. pylori. It did not inhibit the antimicrobial activity of the antibiotics amoxycillin, clarithromycin, erythromycin or tetracycline against H. pylori. The metal ions had inhibitory effects on the antimicrobial activity of amoxycillin, erythromycin and tetracycline, but not on that of clarithromycin.
This study suggests that sofalcone is a suitable candidate for combination therapy.
研究一系列潜在联合疗法抗幽门螺杆菌的作用机制。
在体外测定某些抗溃疡药物对有效抗幽门螺杆菌抗生素抗菌活性的影响。
幽门螺杆菌在斯氏琼脂上培养。使用阿莫西林、克拉霉素、红霉素和四环素。所研究的抗溃疡药物包括氯化铝、索法酮[2'-羧甲基4,4'-双(3-甲基-2-丁烯氧基)查耳酮]和氯化锌。脲酶活性通过脲酶-吲哚酚法测定。通过平板接种法测定最低抑菌浓度，将幽门螺杆菌接种在含有不同浓度抗生素加亚致死剂量抗溃疡药物的平板上。
这项体外研究表明，索法酮具有直接抗菌作用，此外，还能抑制幽门螺杆菌的黏附特性。它不抑制阿莫西林、克拉霉素、红霉素或四环素对幽门螺杆菌的抗菌活性。金属离子对阿莫西林、红霉素和四环素的抗菌活性有抑制作用，但对克拉霉素没有。
本研究表明索法酮是联合疗法的合适候选药物。
