Murakita H, Hirai M, Ito S, Azuma T, Kato T, Kohli Y
Second Department of Internal Medicine, Fukui Medical School, Japan.
Eur J Gastroenterol Hepatol. 1994 Dec;6 Suppl 1:S29-31.
We investigated the production of vacuolating cytotoxin by Helicobacter pylori isolates from patients with peptic ulcer, atrophic gastritis or gastric carcinoma in order to examine the pathophysiological significance of vacuolating cytotoxin in these diseases.
H. pylori was isolated from 18 patients (five with peptic ulcers, seven with atrophic gastritis and six with gastric carcinoma). Culture supernatants of H. pylori isolates, concentrated 20-fold, were serially diluted and then analyzed for cytotoxin activity semi-quantitatively using A431 cells as indicator cells. The relative activity of vacuolating cytotoxin was defined according to the maximum dilution.
Cytotoxin production was observed in two out of five, six out of seven and six out of six isolates from peptic ulcer, atrophic gastritis and gastric carcinoma patients, respectively. The mean relative activity was calculated as 0.80, 2.71 and 2.50 in
These results suggest that vacuolating cytotoxin-producing H. pylori is strongly associated with both atrophic gastritis and gastric carcinoma.
我们研究了从消化性溃疡、萎缩性胃炎或胃癌患者中分离出的幽门螺杆菌产生空泡毒素的情况，以探讨空泡毒素在这些疾病中的病理生理意义。
从18例患者（5例消化性溃疡、7例萎缩性胃炎和6例胃癌患者）中分离出幽门螺杆菌。将幽门螺杆菌分离株的培养上清液浓缩20倍，进行系列稀释，然后以A431细胞作为指示细胞半定量分析细胞毒素活性。根据最大稀释度定义空泡毒素的相对活性。
分别在消化性溃疡、萎缩性胃炎和胃癌患者的5株、7株和6株分离株中，各有2株、6株和6株观察到细胞毒素产生。平均相对活性分别计算为0.80、2.71和2.50。
这些结果表明，产生空泡毒素的幽门螺杆菌与萎缩性胃炎和胃癌均密切相关。
