Kazarian K K, Perdue P W, Lynch W, Dziki A, Nevola J, Lee C H, Hayward I, Williams T, Law W R
Septic Shock Program, Naval Medical Research Institute, Bethesda, Maryland, USA.
Shock. 1994 Mar;1(3):201-12.
The characteristics of two types of intraperitoneal (i.p.) soilage sepsis models, autologous fecal inoculum (FEC) and a pure culture of Escherichia coli (EC), were studied in 26 male Yucatan minipigs (20-30 kg). Early (1-4 h) and late (24-72 h) changes were different between the two groups. The EC group was characterized early by hypotension, low cardiac output, and increased systemic and pulmonary vascular resistances, along with leukopenia, hypoglycemia, lactacidemia, and elevated blood urea nitrogen. Of the pigs in the EC group that survived the early effects, there were few significant differences in physiological parameters, compared to control pigs, that would indicate ongoing pathological processes. In contrast, the FEC group pigs demonstrated early hypotension, but with increased cardiac output and reduced systemic vascular resistance. Other parameter changes were similar to those seen in the EC pigs, but to a lesser degree, with the exception of elevations in serum lactate dehydrogenase. Also in contrast to the EC group, most of the changes in the FEC group persisted in later days, and FEC pigs demonstrated leukocytosis. There were also greater elevations in circulating lipopolysaccharide (LPS) concentrations in the EC group that returned later to baseline levels. In the FEC group, there were persistently elevated LPS concentrations over 72 h. These observations suggest that pigs challenged with intraperitoneal E. coli demonstrated an initial acute peritonitis and damaging physiologic effects of high levels of circulating LPS. Survivors in this group improved and were physiologically stable after 24 h. Pigs that received i.p. autologous feces developed an early acute peritonitis phase with lower levels of circulating LPS, and later developed pronounced peritoneal reaction as demonstrated by multiple abdominal abscesses, pyogenic granuloma formation, and adhesions with physiological evidence of developing sepsis over 72 h. These observations indicate that i.p. EC models evoke a systemic response not unlike intravenous administration of LPS or EC, however, the FEC model produced a systemic response akin to a slower developing septic process.
在26头体重20 - 30千克的雄性尤卡坦小型猪中，研究了两种腹腔内（i.p.）污染性脓毒症模型的特征，即自体粪便接种（FEC）模型和大肠杆菌纯培养物（EC）模型。两组在早期（1 - 4小时）和晚期（24 - 72小时）的变化有所不同。EC组早期表现为低血压、心输出量降低、全身和肺血管阻力增加，同时伴有白细胞减少、低血糖、乳酸血症和血尿素氮升高。在EC组中，那些在早期影响中存活下来的猪，与对照猪相比，生理参数几乎没有显著差异表明存在持续的病理过程。相比之下，FEC组的猪早期表现为低血压，但心输出量增加且全身血管阻力降低。其他参数变化与EC组猪相似，但程度较轻，血清乳酸脱氢酶升高除外。同样与EC组不同的是，FEC组的大多数变化在后期持续存在，且FEC组猪表现出白细胞增多。此外，EC组循环脂多糖（LPS）浓度升高幅度更大，随后恢复到基线水平。在FEC组中，LPS浓度在72小时内持续升高。这些观察结果表明，腹腔内接种大肠杆菌的猪表现出最初的急性腹膜炎以及高水平循环LPS的有害生理效应。该组中的存活猪在24小时后情况改善且生理稳定。接受腹腔内自体粪便接种的猪出现早期急性腹膜炎阶段，循环LPS水平较低，随后出现明显的腹膜反应，表现为多个腹部脓肿、化脓性肉芽肿形成以及粘连，并有超过72小时的脓毒症发展的生理证据。这些观察结果表明，腹腔内EC模型引发的全身反应与静脉注射LPS或EC并无不同，然而，FEC模型产生的全身反应类似于发展较慢的脓毒症过程。
