Schoeller D A, Taylor P B, Shay K
Committee on Human Nutrition and Nutritional Biology, University of Chicago, IL 60637, USA.
Obes Res. 1995 Mar;3 Suppl 1:15-20. doi: 10.1002/j.1550-8528.1995.tb00003.x.
The doubly labeled water method is the first method that accurately measures total daily energy expenditure in free-living subjects over periods of days to weeks. Validations have indicated that the method can be performed with a coefficient of variation of between 3% and 5%. This precision, however, is dependent on the quality of the isotopic analyses. A recent interlaboratory comparison has indicated that there is a wide variation in the accuracy and precision with which deuterium and 18O enrichments are measured. This reduces the accuracy and precision with which a laboratory will perform the doubly labeled water method and in some cases may limit the application of this technique. Herein we review the analytical requirements for optimal use of the method and some of the potential sources of error in the stable isotope analysis.
双标记水法是第一种能够在数天至数周的时间内准确测量自由活动受试者每日总能量消耗的方法。验证表明，该方法的变异系数在3%至5%之间。然而，这种精度取决于同位素分析的质量。最近的一项实验室间比较表明，在测量氘和18O富集度时，准确性和精度存在很大差异。这降低了实验室进行双标记水法的准确性和精度，在某些情况下可能会限制该技术的应用。在此，我们回顾了该方法最佳使用的分析要求以及稳定同位素分析中一些潜在的误差来源。
