Tamai M, Tanimura H, Yamaue H, Iwahashi M, Tsunoda T, Tani M, Noguchi K, Mizobata S, Hotta T, Arii K
Department of Gastroenterological Surgery, Wakayama Medical School, Japan.
Cancer Lett. 1995 Apr 14;90(2):111-7. doi: 10.1016/0304-3835(95)03695-s.
The expression of carcinoembryonic antigen(CEA) on tumor cells freshly excised from 51 patients with gastric cancer was studied using flow cytometry. The expression of CEA by flow cytometry was more quantitative than that by immunohistochemical staining. There was no relationship between the fluorescence intensity assessed by flow cytometry and serum CEA levels, except for patients with a high titer of serum CEA. The patients with high grade CEA expression on tumor cells by flow cytometry had poor prognoses, compared to patients with low CEA expression in undifferentiated gastric cancer. Thus, it is suggested that the quantitative CEA expression on tumor cells by flow cytometry could be a useful prognostic marker in postoperative gastric cancer patients.
采用流式细胞术研究了51例胃癌患者新鲜切除肿瘤细胞上癌胚抗原（CEA）的表达情况。流式细胞术检测CEA的表达比免疫组织化学染色更具定量性。除血清CEA高滴度的患者外，流式细胞术评估的荧光强度与血清CEA水平之间无相关性。与未分化胃癌中CEA低表达的患者相比，流式细胞术检测肿瘤细胞上CEA高表达的患者预后较差。因此，提示流式细胞术检测肿瘤细胞上CEA的定量表达可能是胃癌术后患者有用的预后标志物。
