Tamai M, Tanimura H, Yamaue H, Iwahashi M, Tsunoda T, Tani M, Noguchi K, Hotta T, Arii K
Department of Gastroenterological Surgery, Wakayama Medical College, Japan.
J Surg Oncol. 1993 Mar;52(3):176-80. doi: 10.1002/jso.2930520312.
The expression of carcinoembryonic antigen (CEA) by tumor cells from freshly excised human gastric cancers was investigated using flow cytometry (FCM). Highly purified fresh human cancer cells were obtained from solid tumors in 20 patients and from malignant ascites in 6 patients. Thirteen of the 26 tumors were positive for CEA by FCM. CEA expression was more common in well-differentiated tumors than in poorly differentiated tumors. CEA expression was investigated by both FCM and immunohistochemistry in 9 patients, and the two methods agreed in 8 of them. However, quantitative evaluation of CEA expression could only be performed by FCM and not by immunohistochemical staining. FCM could analyze the expression of CEA not only on the cell membrane but also in the cytoplasm, by using gastric cancer cells with or without Triton X-100 treatment. Thus, this study showed that CEA expression can be determined and evaluated quantitatively by FCM.
采用流式细胞术（FCM）对新鲜切除的人类胃癌肿瘤细胞中癌胚抗原（CEA）的表达进行了研究。从20例患者的实体瘤和6例患者的恶性腹水中获取了高度纯化的新鲜人类癌细胞。26个肿瘤中有13个通过FCM检测CEA呈阳性。CEA表达在高分化肿瘤中比在低分化肿瘤中更常见。对9例患者同时采用FCM和免疫组织化学方法研究CEA表达，其中8例两种方法结果一致。然而，CEA表达的定量评估只能通过FCM进行，而不能通过免疫组织化学染色进行。通过对有无Triton X-100处理的胃癌细胞进行检测，FCM不仅可以分析细胞膜上的CEA表达，还可以分析细胞质中的CEA表达。因此，本研究表明FCM可以对CEA表达进行定量测定和评估。
