1α,25 - 二羟基维生素D3诱导小鼠红白血病细胞分化

Induction of differentiation in murine erythroleukemia cells by 1 alpha,25-dihydroxy vitamin D3.

作者信息

Radhika S, Choudhary S K, Garg L C, Dixit A

机构信息

Department of Zoology, University of Delhi, India.

出版信息

Cancer Lett. 1995 Apr 14;90(2):225-30. doi: 10.1016/0304-3835(95)03707-4.

DOI:10.1016/0304-3835(95)03707-4

PMID:7736459

Abstract

The Friend murine erythroleukemia (MEL) cells can be stimulated to differentiate in response to a variety of chemical inducing agents. In the present study, the effect of 1 alpha,25-dihydroxyvitamin D3 on differentiation of MEL cells was investigated. Vitamin D3 induced differentiation of MEL cells in culture as determined by elevated hemoglobin content, a rise in the number of benzidine-positive cells and increase in acetylcholine esterase activity. The optimum concentration of the vitamin required to induce differentiation of MEL cells was found to be 750 nM. The pattern of induction of differentiation was similar to that observed with DMSO and the induction of differentiation by vitamin D3 was inhibited by dexamethasone.

摘要

Friend小鼠红白血病（MEL）细胞可被多种化学诱导剂刺激而发生分化。在本研究中，研究了1α,25 - 二羟基维生素D3对MEL细胞分化的影响。通过提高血红蛋白含量、增加联苯胺阳性细胞数量以及提高乙酰胆碱酯酶活性来确定，维生素D3可诱导培养中的MEL细胞分化。发现诱导MEL细胞分化所需的维生素最佳浓度为750 nM。分化诱导模式与用二甲基亚砜（DMSO）观察到的相似，并且维生素D3诱导的分化受到地塞米松的抑制。