Diederen J H, Vullings H G
Department of Experimental Zoology, Utrecht University, The Netherlands.
Cell Tissue Res. 1995 Mar;279(3):585-90. doi: 10.1007/BF00318170.
The influence of flight activity on the formation of secretory granules and the concomitant membrane recycling by the trans-Golgi network in the peptidergic neurosecretory adipokinetic cells of Locusta migratoria was investigated by means of ultrastructural morphometric methods. The patterns of labelling of the trans-Golgi network by the exogenous adsorptive endocytotic tracer wheat-germ agglutinin-conjugated horse-radish peroxidase and by the endogenous marker enzyme acid phosphatase were used as parameters and were measured by an automatic image analysis system. The results show that endocytosed fragments of plasma membrane with bound peroxidase label were transported to the trans-Golgi network and used to build new secretory granules. The amounts of peroxidase and especially of acid phosphatase within the trans-Golgi network showed a strong tendency to be smaller in flight-stimulated cells than in non-stimulated cells. The amounts of acid phosphatase in the immature secretory granules originating from the trans-Golgi network were significantly smaller in stimulated cells. The number of immature secretory granules positive for acid phosphatase tended to be higher in stimulated cells. Thus, flight stimulation of adipokinetic cells for 1 h influences the functioning of the trans-Golgi network; this most probably results in a slight enhancement of the production of secretory granules by the trans-Golgi network.
通过超微结构形态计量学方法，研究了飞行活动对飞蝗肽能神经分泌性脂肪动激素细胞中分泌颗粒形成以及反式高尔基体网络伴随的膜循环的影响。以外源吸附性内吞示踪剂小麦胚凝集素结合辣根过氧化物酶和内源性标记酶酸性磷酸酶对反式高尔基体网络的标记模式作为参数，并通过自动图像分析系统进行测量。结果表明，带有结合过氧化物酶标记的质膜内吞片段被转运至反式高尔基体网络，并用于构建新的分泌颗粒。反式高尔基体网络中过氧化物酶尤其是酸性磷酸酶的含量在飞行刺激的细胞中比未刺激的细胞有明显减少的趋势。源自反式高尔基体网络的未成熟分泌颗粒中酸性磷酸酶的含量在受刺激细胞中显著减少。酸性磷酸酶阳性的未成熟分泌颗粒数量在受刺激细胞中趋于增加。因此，对脂肪动激素细胞进行1小时的飞行刺激会影响反式高尔基体网络的功能；这很可能导致反式高尔基体网络分泌颗粒的产生略有增加。
