Kashiwabuchi N, Ikeda K, Araki K, Hirano T, Shibuki K, Takayama C, Inoue Y, Kutsuwada T, Yagi T, Kang Y
Department of Neuropharmacology, Niigata University, Japan.
Cell. 1995 Apr 21;81(2):245-52. doi: 10.1016/0092-8674(95)90334-8.
Of the six glutamate receptor (GluR) channel subunit families identified by molecular cloning, five have been shown to constitute either the AMPA, kainate, or NMDA receptor channel, whereas the function of the delta subunit family remains unknown. The selective localization of the delta 2 subunit of the GluR delta subfamily in cerebellar Purkinje cells prompted us to examine its possible physiological roles by the gene targeting technique. Analyses of the GluR delta 2 mutant mice reveal that the delta 2 subunit plays important roles in motor coordination, formation of parallel fiber-Purkinje cell synapses and climbing fiber-Purkinje cell synapses, and long-term depression of parallel fiber-Purkinje cell synaptic transmission. These results suggest a close relationship between synaptic plasticity and synapse formation in the cerebellum.
通过分子克隆鉴定出的六个谷氨酸受体（GluR）通道亚基家族中，有五个已被证明构成AMPA、海人藻酸或NMDA受体通道，而δ亚基家族的功能仍然未知。GluR δ亚家族的δ2亚基在小脑浦肯野细胞中的选择性定位促使我们通过基因靶向技术研究其可能的生理作用。对GluR δ2突变小鼠的分析表明，δ2亚基在运动协调、平行纤维 - 浦肯野细胞突触和攀缘纤维 - 浦肯野细胞突触的形成以及平行纤维 - 浦肯野细胞突触传递的长时程抑制中起重要作用。这些结果表明小脑突触可塑性和突触形成之间存在密切关系。
