Gilson P R, Adcock G J, Howlett B J, McFadden G I
Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria, Australia.
Curr Genet. 1995 Feb;27(3):239-42. doi: 10.1007/BF00326155.
Southern hybridisation, polymerase chain reaction (PCR), and nucleotide sequence, data indicate that the 5s ribosomal RNA (rRNA) gene is linked to the main rRNA gene repeat in the nuclear genome of four cryptomonad algae (Rhinomonas pauca, Storeatula major, Komma caudata, and isolate Cs 134). The 5s gene is apparently transcribed in the same direction as the large and small subunit rRNA genes. The intergenic spacer between the 5s gene and the large subunit rRNA gene exhibits length and sequence polymorphism among the different species. Cryptomonads contain two different eukaryotic genomes: the host nucleus and the nucleus of a eukaryotic endosymbiont. Mapping experiments with isolated chromosomes of the host and endosymbiont genomes showed that the intergenic spacer between the large subunit and the 5s rRNA gene, which was amplified from total DNA by PCR, was derived from the host nuclear genome.
Southern杂交、聚合酶链反应（PCR）和核苷酸序列数据表明，5S核糖体RNA（rRNA）基因与四种隐藻（少角鼻隐藻、大贮环隐藻、尾状科玛隐藻和Cs 134分离株）核基因组中的主要rRNA基因重复序列相连。5S基因显然与大、小亚基rRNA基因转录方向相同。5S基因与大亚基rRNA基因之间的基因间隔区在不同物种间呈现长度和序列多态性。隐藻含有两种不同的真核基因组：宿主细胞核和真核内共生体的细胞核。对宿主和内共生体基因组的分离染色体进行的定位实验表明，通过PCR从总DNA中扩增出的大亚基与5S rRNA基因之间的基因间隔区来自宿主核基因组。
