Rizzu P, Baldini A
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.
Cytogenet Cell Genet. 1995;70(1-2):26-8. doi: 10.1159/000133984.
While constructing a contig in the human chromosome region 3q27, we identified two YAC clones that were positive for the polymorphic marker D3S1602. One of these clones was also positive for a sequence-tagged site derived from the kininogen (KNG) gene. Because of the known evolutionary and structural relationship of KNG to other members of the cystatin gene superfamily, we tested the physical linkage of the genes encoding alpha-2HS-glycoprotein (AHSG), KNG, and histidine-rich glycoprotein (HRG), all of which were previously mapped to the long arm of chromosome 3. Our results show the colocalization of the three genes in two independent, partially overlapping YAC clones. The genomic inserts of the two clones were 1 Mb and 1.3 Mb in size, indicating that the three genes map within 1 Mb of DNA. The largest YAC was also positive for the polymorphic marker D3S1262, substantiating previously reported data of genetic linkage between this marker and HRG. Fluorescence in situ hybridization localized the two YAC clones to chromosome band 3q27.
在构建人类染色体3q27区域的重叠群时，我们鉴定出两个对多态性标记D3S1602呈阳性的酵母人工染色体（YAC）克隆。其中一个克隆对源自激肽原（KNG）基因的序列标签位点也呈阳性。由于已知KNG与胱抑素基因超家族其他成员之间的进化和结构关系，我们检测了编码α-2HS-糖蛋白（AHSG）、KNG和富含组氨酸糖蛋白（HRG）的基因的物理连锁，所有这些基因先前都被定位到染色体3的长臂上。我们的结果显示这三个基因在两个独立的、部分重叠的YAC克隆中共定位。这两个克隆的基因组插入片段大小分别为1兆碱基（Mb）和1.3 Mb，表明这三个基因位于1 Mb的DNA范围内。最大的YAC对多态性标记D3S1262也呈阳性，证实了先前报道的该标记与HRG之间的遗传连锁数据。荧光原位杂交将这两个YAC克隆定位到染色体带3q27。
