Hughes K J, Everiss K D, Kovach M E, Peterson K M
Department of Microbiology and Immunology, Louisiana State University Medical Center-Shreveport 71130, USA.
Gene. 1995 Apr 14;156(1):59-61. doi: 10.1016/0378-1119(95)00054-a.
The nucleotide sequence of the Vibrio cholerae acfA gene (encoding an accessory colonization factor) has been determined. Sequence analysis revealed the presence of an open reading frame of 215 amino acids with a characteristic signal peptidase I (SPI) cleavage site at the N terminus. Electrophoretic analysis of proteins synthesized by Escherichia coli cells, following T7 promoter/RNA polymerase-directed expression of acfA, revealed a 23-kDa protein corresponding to the mature form of AcfA. The T7 expression system also showed that, in the presence of known SPI inhibitors, a 25-kDa unprocessed form of AcfA is produced.
霍乱弧菌acfA基因（编码一种辅助定植因子）的核苷酸序列已被确定。序列分析显示存在一个由215个氨基酸组成的开放阅读框，其N端有一个特征性的信号肽酶I（SPI）切割位点。在T7启动子/RNA聚合酶指导下表达acfA后，对大肠杆菌细胞合成的蛋白质进行电泳分析，发现了一种与AcfA成熟形式相对应的23 kDa蛋白质。T7表达系统还表明，在存在已知SPI抑制剂的情况下，会产生一种25 kDa未加工形式的AcfA。
