Harkey C W, Everiss K D, Peterson K M
Department of Microbiology and Immunology, Louisiana State University Medical Center-Shreveport 71130-3932.
Gene. 1995 Feb 3;153(1):81-4. doi: 10.1016/0378-1119(94)00811-6.
The Vibrio cholerae (Vc) gene (tagA) coding for the TagA lipoprotein has been isolated. Sequencing of tagA revealed the presence of an open reading frame (ORF) of 568 amino acids with a characteristic signal peptidase II cleavage site at the N terminus. Electrophoretic analysis of proteins synthesized by Escherichia coli (Ec) cells following T7 promoter/RNA polymerase-directed expression of tagA, revealed a closely migrating doublet of proteins corresponding to two species of TagA. Computer-generated alignment algorithms predict that a homology exists between Vc TagA and Ec K99 fimbriae biogenesis determinant FanD.
编码TagA脂蛋白的霍乱弧菌（Vc）基因（tagA）已被分离出来。tagA测序显示存在一个由568个氨基酸组成的开放阅读框（ORF），其N端有一个特征性的信号肽酶II切割位点。在T7启动子/RNA聚合酶指导下表达tagA后，对大肠杆菌（Ec）细胞合成的蛋白质进行电泳分析，发现有一对紧密迁移的双峰蛋白，对应于两种TagA。计算机生成的比对算法预测，Vc TagA与Ec K99菌毛生物合成决定因子FanD之间存在同源性。
