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[大肠杆菌K-12 recA菌株抗紫外线回复突变体的分离与特性]

[Isolation and characteristics of UV-resistant revertants of recA-strains of Escherichia coli K-12].

作者信息

Torosian M V, Shishkova O V, Rabinkova E V

出版信息

Genetika. 1975;10(9):123-32.

PMID:773753
Abstract

The paper presents the results of a study of UV-resistant revertants of some recA strains induced by different mutagens. It is shown that some of produced revertants differ from original recA strains in some properties. It is established that all UV-resistant revertant fall into three phenotypic groups on their recombination proficiency in crosses with different donor strains (Hfr C, Hfr 3.0SO, F' W1655) and in their sensitivity to UV and gamma-rays. It is concluded that all UVr revertants are rec A+ and carried mutations either in the same recA gene (true reversion or intragenic suppression) or in genes closely linked with recA.

摘要

本文介绍了用不同诱变剂诱导某些recA菌株产生抗紫外线回复突变体的研究结果。结果表明,一些产生的回复突变体在某些特性上与原始recA菌株不同。已经确定,所有抗紫外线回复突变体在与不同供体菌株(Hfr C、Hfr 3.0SO、F'W1655)杂交时的重组能力以及对紫外线和γ射线的敏感性方面可分为三个表型组。得出的结论是,所有抗紫外线回复突变体都是rec A+,并且在同一个recA基因中发生了突变(真正的回复突变或基因内抑制),或者在与recA紧密连锁的基因中发生了突变。

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