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[大肠杆菌K-12 recA菌株的回复突变体]

[Revertants of recA-strains of Escherichia coli K-12].

作者信息

Shishkova O V, Torosian M V, Rabinkova E V

出版信息

Genetika. 1976;12(1):98-110.

PMID:773764
Abstract

The paper presents the results of a study of revertants of strains AB2463 recA13, AB2465 rec15, No 41 rec16. It is shown that revertants are characterized as intermediate strains between recA and rec+ (on the level of recB, recC strains) on their recombination proficiency in crosses with Hfr, sensitivity to UV and gamma-rays and in F-heterogenote formed cultures on their capacity of the formation of recombinants between episome and chromosome and the capacity to chromosome mobilization. In the same time revertants do not differ from rec+ in the frequency of spontaneous and UV-induction of prophage constitution of recombinants formed in crosses, and in the synthesis of beta-galactosidase in postconjugation period. It is found that the mutations studied are mapped apparently in recA gene.

摘要

本文展示了对AB2463 recA13、AB2465 rec15、41号rec16菌株回复突变体的研究结果。结果表明,回复突变体在与高频重组(Hfr)进行杂交时的重组能力、对紫外线和γ射线的敏感性以及在F-异源合子形成培养物中附加体与染色体之间形成重组体的能力和染色体动员能力方面,表现为recA和rec+之间的中间菌株(在recB、recC菌株水平上)。同时,回复突变体在杂交中形成重组体的原噬菌体自发形成频率和紫外线诱导频率以及接合后β-半乳糖苷酶的合成方面与rec+没有差异。研究发现,所研究的突变显然定位于recA基因中。

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