[从大肠杆菌K-12 recA基因突变培养物中分离得到的rec⁺回复突变体的性质]
[Nature of rec+ revertants isolated from E. coli K-12 cultures mutant with regard to gene rec A].
作者信息
Naumova O B
出版信息
Biull Eksp Biol Med. 1976 Dec;82(12):1487-8.
The nature of Rec+ revertants isolated from the E. coli K-12 AB 2463 recA13 defective strain cultures was ascertained by transduction of the recA gene region into the cells of the JC 2915F- strain with the aid of P1 vira phage. Then the recombination capacity of the transductants was tested by crossing with the JC 158 Hfr strain, and the UV sensitivity of the transductants was determined. Besides, the response of the transductants to the suppression phages was examined. As revealed, the REc+ revertants were characterized by differences in the recA gene. In a number of Rec+ revertants phenotype Rec+ appeared as a result of reverse mutation in this gene from rec- to rec+, whereas in other revertants Rec+ phenotype was due to indirect suppression.
从大肠杆菌K-12 AB 2463 recA13缺陷型菌株培养物中分离出的Rec⁺回复突变体的性质，是通过借助P1噬菌体将recA基因区域转导到JC 2915F⁻菌株的细胞中来确定的。然后通过与JC 158 Hfr菌株杂交来测试转导子的重组能力，并测定转导子对紫外线的敏感性。此外，还检测了转导子对抑制噬菌体的反应。结果表明，Rec⁺回复突变体的recA基因存在差异。在一些Rec⁺回复突变体中，Rec⁺表型是由于该基因从rec⁻反向突变为rec⁺所致，而在其他回复突变体中，Rec⁺表型则是由于间接抑制作用。
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