Vasiunina E A, Poslovina A S, Andreeva I S, Salganik R I
Genetika. 1975;11(3):127-32.
The effect of some adenyl precurosors of DNA synthesis on the mutagenic activity oh hydroxylamine (HA) is studied. It is shown that the addition of adenine to a suspension of Escherichia coli Bcells increases the yield of mutants by more than two times as compared with HA alone. The effects of adenosine, AMP and dAMP are somewhat different. It is suggested that the increase of the HAmutagenic effect produced by the addition of adenine may be due to:1) the excess of the amount of adenylic precursors of DNA synthesis over guanilic ones, which promotes the erroneous base-pairing during the replication of the HA modified template; 2) the modification of adenylic precursors by HA into N6-oxy-dATP, and their incorporation into DNA. The mutagenic effect of N6-hydroxyadenosine, the product of the adenine modification by HA, in E. coli Bpur- was studied. The experiments showed that N6-hydroxyadenosine induced about 1% of mutations, a relatively low lethal effect (the cell survival was 80%), and provided a high mutagenic action of this compound.
研究了一些DNA合成的腺苷前体对羟胺（HA）诱变活性的影响。结果表明，向大肠杆菌B细胞悬液中添加腺嘌呤，与单独使用HA相比，突变体产量增加了两倍多。腺苷、AMP和dAMP的作用略有不同。有人认为，添加腺嘌呤导致HA诱变效应增加可能是由于：1）DNA合成的腺苷前体数量超过鸟苷前体，这促进了HA修饰模板复制过程中的错误碱基配对；2）HA将腺苷前体修饰为N6-氧代-dATP，并将其掺入DNA。研究了HA对腺嘌呤修饰产物N6-羟基腺苷在大肠杆菌Bpur-中的诱变作用。实验表明，N6-羟基腺苷诱导约1%的突变，致死效应相对较低（细胞存活率为80%），且该化合物具有较高的诱变作用。
