[细菌基因recB、recA、exr和polA对大肠杆菌溶菌酶原生质体T4噬菌体DNA转染的影响]
[The influence of bacterial genes recB, recA, exr and polA on T4 phage DNA transfection of lysozyme spheroplasts of Escherichia coli].
作者信息
Nazarova A F
出版信息
Genetika. 1976;12(1):116-21.
PMID:773763
Abstract
The transfection efficiency of T4 phage DNA of Escherichia coli spheroplasts, carrying mutations in four genes, involved in recombination and reparation (recB, recA, exr, polA), is studied. RecB- and recA- mutations increased the phage yield during transfection, and exr- and polA- mutations decreased it. RecA- mutation is suggested to influence on spheroplasts membrane state, resulting in more efficient replication of transfecting phage DNA.
摘要
对携带参与重组和修复的四个基因（recB、recA、exr、polA）发生突变的大肠杆菌原生质体的T4噬菌体DNA转染效率进行了研究。RecB和recA突变增加了转染过程中的噬菌体产量，而exr和polA突变则降低了产量。有人认为RecA突变会影响原生质体膜的状态，从而使转染的噬菌体DNA更有效地复制。
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