Unadkat P, Newman B, Tedder R S
Division of Virology, University College London Medical School, UK.
J Virol Methods. 1995 Feb;51(2-3):145-52. doi: 10.1016/0166-0934(94)00096-y.
A rapid and simultaneous competitive enzyme immunoassay (EIA) for the detection of human antibodies to varicella zoster virus (VZV) which utilises complement fixing (CF) VZV antigen and peroxidase labelled human antibodies to VZV as reagents is described. The competitive EIA was compared with competitive radioimmunoassay (RIA) in a selection of sera from 501 blood donors and 115 patients. The competitive EIA was comparable in sensitivity and specificity to the RIA, but had the advantage of not requiring radiolabelling or facilities for radioactive handling and disposal, and also had an enhanced stability. The competitive EIA was compared with competitive RIA, a commercially available latex agglutination assay and an antiglobulin type EIA on sera from 100 patients. The competitive EIA and RIA appeared to be the most sensitive tests.
本文描述了一种快速同步竞争酶免疫测定法（EIA），用于检测人抗水痘带状疱疹病毒（VZV）抗体，该方法使用补体结合（CF）VZV抗原和过氧化物酶标记的人抗VZV抗体作为试剂。在501名献血者和115名患者的一组血清样本中，将竞争EIA与竞争放射免疫测定法（RIA）进行了比较。竞争EIA在敏感性和特异性方面与RIA相当，但具有无需放射性标记以及放射性处理和处置设施的优势，并且稳定性更强。在100名患者的血清样本中，将竞争EIA与竞争RIA、一种市售乳胶凝集试验和一种抗球蛋白类型EIA进行了比较。竞争EIA和RIA似乎是最敏感的检测方法。
