Wreghitt T G, Tedder R S, Nagington J, Ferns R B
J Med Virol. 1984;13(4):361-70. doi: 10.1002/jmv.1890130407.
The competitive ELISA test was compared with competitive RIA, complement fixation, and indirect fluorescent antibody test for the detection of antibody to varicella-zoster virus (VZV). ELISA and RIA were the most sensitive tests, being tenfold as sensitive as indirect immunofluorescence and 20 times as sensitive as complement fixation, which was surprisingly inadequate for detecting anti-VZV in sera from regular haemodialysis patients. The ELISA test was found to be the most suitable assay, employing a routine complement fixation test (CFT) grade viral antigen and not requiring radiolabelling and counting facilities, which are unavailable in most virology laboratories.
为检测水痘-带状疱疹病毒（VZV）抗体，将竞争性酶联免疫吸附测定（ELISA）试验与竞争性放射免疫测定（RIA）、补体结合试验和间接荧光抗体试验进行了比较。ELISA和RIA是最敏感的试验，其敏感性是间接免疫荧光的10倍，是补体结合试验的20倍，而补体结合试验在检测常规血液透析患者血清中的抗VZV时出人意料地不够灵敏。ELISA试验被认为是最合适的检测方法，它采用常规补体结合试验（CFT）级别的病毒抗原，且不需要放射性标记和计数设备，而大多数病毒学实验室都没有这些设备。
