Litchfield W J, Wells W W
Infect Immun. 1976 Mar;13(3):728-34. doi: 10.1128/iai.13.3.728-734.1976.
To account for enhanced susceptibility to infection among galactosemics, the acute effects of D-galactose on metabolic and functional activities of phagocytic cells in vitro were investigated. Human and guinea pig polymorphonuclear leukocytes (PMN) when incubated in medium containing 30 mM galactose displayed substantially less killing of Escherichia coli than when incubated in medium with 5 mM glucose. Impaired bactericidal activity was dependent upon galactose concentration but could be partially averted by supplementing the galactose-containing medium with 15 mM glucose. Phagocytic activities of guinea pig PMN and peritoneal macrophages were assayed by following ingestion of 32P-labeled E. coli and were also depressed by elevated galactose. Galactose was readily epimerized to glucose by resting PMN, and this conversion was stimulated by phagocytosis. Incubation of macrophages in the presence of galactose resulted in depletion of intracellular levels of adenosine 5' -triphosphate as well as other metabolities.
为了解释半乳糖血症患者对感染易感性增强的原因，我们研究了D-半乳糖对体外吞噬细胞代谢和功能活性的急性影响。当人及豚鼠的多形核白细胞（PMN）在含有30 mM半乳糖的培养基中孵育时，其对大肠杆菌的杀伤作用明显低于在含有5 mM葡萄糖的培养基中孵育时。杀菌活性受损取决于半乳糖浓度，但通过在含半乳糖的培养基中添加15 mM葡萄糖可部分避免。通过追踪32P标记的大肠杆菌的摄取来检测豚鼠PMN和腹腔巨噬细胞的吞噬活性，结果显示半乳糖水平升高也会使其降低。静息的PMN可轻易地将半乳糖差向异构化为葡萄糖，并且这种转化会因吞噬作用而受到刺激。在半乳糖存在的情况下孵育巨噬细胞会导致细胞内三磷酸腺苷以及其他代谢产物水平的消耗。
