Domer J E
Infect Immun. 1976 Mar;13(3):790-9. doi: 10.1128/iai.13.3.790-799.1976.
Guinea pigs were infected with different doses of yeasts of Histoplasma capsulatum or artifically immunized with several concentrations of unextracted yeast cell walls, and then tested in vivo and in vitro for cell-mediated responses to various subcellular fractions of the fungus. Three types of cell-mediated responses were measured, viz., skin test activity, production of migration inhibition factor, and lymphocyte transformation. Positive cutaneous reactions were elicited in animals immunized with 100 or 1,000 mug of cell walls when such animals were skin-tested with cell wall glycoprotein of soluble cytoplasmic substances, whereas animals immunized with 2,000 mug of cell walls did not react significantly greater than unsensitized animals when skin-tested with the same antigens. Histoplasmin did not elicit cutaneous sensitivity in guinea pigs infected with the smallest inoculum, 6 X 10(5) yeast cells, or in animals immunized with cell walls, regardless of the concentration of cell walls used as immunogen. However, hypersensitivity to H. capsulatum could be detected with cytoplasmic substances in animals infected with 6X 10(5). In guinea pigs infected with larger doses, i.e., 10 X 10(7), 15 X10(7), or 20 X 10(7), hypersensitivity could be detected with histoplasmin, cell wall glycoprotein, a ribosome-rich fraction, and soluble cytoplasmic substances. Both cell wall glycoprotein and soluble cytoplasmic substances were functional in migration inhibition factor assays with peritoneal exudate cells from animals immunized with 100 or 1,000 mug of cell walls. The transformation of lymphocytes from infected and artificially immunized guinea pigs in the presence of cell wall glycoprotein and soluble cytoplasmic substances was variable and unpredictable, the lymphocytes from some animls within a given group transforming and those from other animals showing no evidence of stimulation. Moreover, the level of stimulation could not be correlated with the degree of dermal hypersensitivity. These findings suggest that cell wall glycoprotein, and the fractions containing ribosomes and soluble cytoplasmic substances, could be useful antigens in assays for cellular immunity, and warrant further investigation with respect to specificity and active components.
将豚鼠用不同剂量的荚膜组织胞浆菌酵母感染，或用几种浓度的未提取酵母细胞壁进行人工免疫，然后在体内和体外测试其对该真菌各种亚细胞组分的细胞介导反应。测量了三种类型的细胞介导反应，即皮肤试验活性、迁移抑制因子的产生和淋巴细胞转化。当用100或1000微克细胞壁免疫的动物用细胞壁糖蛋白或可溶性细胞质物质进行皮肤试验时，会引发阳性皮肤反应，而当用相同抗原进行皮肤试验时，用2000微克细胞壁免疫的动物与未致敏动物相比，反应并无显著增强。组织胞浆菌素在感染最小接种量（6×10⁵个酵母细胞）的豚鼠中，或在用细胞壁免疫的动物中，无论用作免疫原的细胞壁浓度如何，均未引发皮肤敏感性。然而，在用6×10⁵个酵母细胞感染的动物中，可通过细胞质物质检测到对荚膜组织胞浆菌的超敏反应。在感染较大剂量（即10×10⁷、15×10⁷或20×10⁷）的豚鼠中，可通过组织胞浆菌素、细胞壁糖蛋白、富含核糖体的组分和可溶性细胞质物质检测到超敏反应。细胞壁糖蛋白和可溶性细胞质物质在用100或1000微克细胞壁免疫的动物的腹膜渗出细胞的迁移抑制因子试验中均有作用。在细胞壁糖蛋白和可溶性细胞质物质存在的情况下，来自感染和人工免疫豚鼠的淋巴细胞转化情况各不相同且不可预测，给定组内一些动物的淋巴细胞发生转化，而其他动物的淋巴细胞则未显示出刺激迹象。此外，刺激水平与皮肤超敏反应程度无关。这些发现表明，细胞壁糖蛋白以及含有核糖体和可溶性细胞质物质的组分，可能是细胞免疫测定中有用的抗原，并且在特异性和活性成分方面值得进一步研究。
