霍乱弧菌O139起源的研究：可能的祖先菌株的鉴定。

Studies on the genesis of Vibrio cholerae O139: identification of probable progenitor strains.

作者信息

Pajni S, Sharma C, Bhasin N, Ghosh A, Ramamurthy T, Nair G B, Ramajayam S, Das B, Kar S, Roychowdhury S

机构信息

Institute of Microbial Technology, Chandigarh, India.

出版信息

J Med Microbiol. 1995 Jan;42(1):20-5. doi: 10.1099/00222615-42-1-20.

DOI:10.1099/00222615-42-1-20

PMID:7739019

Abstract

Four lines of evidence suggest that the recent outbreak strains of Vibrio cholerae O139 could have emerged from serogroup O1 strains typified by isolates M01 and M0477 described in this paper, which are neither truly classical nor truly E1 Tor in their biotype attributes. Firstly, like all O139 isolates, these O1 strains, isolated in Madras during and before the O139 outbreak, were resistant not only to polymyxin B but also to all biotype-specific choleraphages, i.e. classical phage phi 149 and E1 Tor phages e4 and e5. Secondly, the restriction fragment pattern (RFP) polymorphism displayed by these strains for the cholera toxin (ctx) gene, were identical with those produced by O139 isolates but were different from those of O1 type strains, namely V. cholerae 569B (classical) and V. cholerae MAK757 (E1 Tor). Thirdly, all the O139 isolates and the two O1 isolates carried an identical large number of copies of cholera toxin gene in their chromosomes. Finally, the outer-membrane protein profiles of strains M01 and M0477 were identical to those of O139 isolates but were different from those displayed by strains 569B and MAK757.

摘要

四条证据表明，霍乱弧菌O139近期的暴发菌株可能源自本文所述的以分离株M01和M0477为代表的O1血清群菌株，这些菌株在生物型属性上既不是真正的古典生物型，也不是真正的埃尔托生物型。首先，与所有O139分离株一样，这些在O139暴发期间及之前于马德拉斯分离出的O1菌株，不仅对多粘菌素B耐药，而且对所有生物型特异性霍乱噬菌体耐药，即古典噬菌体phi 149以及埃尔托噬菌体e4和e5。其次，这些菌株霍乱毒素（ctx）基因所显示的限制性片段模式（RFP）多态性，与O139分离株产生的模式相同，但与O1型菌株，即霍乱弧菌569B（古典生物型）和霍乱弧菌MAK757（埃尔托生物型）的模式不同。第三，所有O139分离株和这两个O1分离株在其染色体中携带数量相同的大量霍乱毒素基因拷贝。最后，菌株M01和M0477的外膜蛋白谱与O139分离株的相同，但与菌株569B和MAK757所显示的不同。