Mukuria C J, Noguchi A, Suzuki E, Naiki M
Department of Veterinary Science, National Institute of Health, Tokyo.
Jpn J Med Sci Biol. 1994 Oct-Dec;47(5-6):253-64. doi: 10.7883/yoken1952.47.253.
A human Hanganutziu-Deicher (HD) antibody and a chicken anti-N-glycolyneuroaminyllactosylceramide (HD3) antibody were compared in their reaction against HD antigen-active ganglioside (HD3) and a glycoprotein (GP) by radioimmunoassay (RIA). The human antibody had a 50 times higher reactivity with the glycoprotein, while the chicken antibody reacted equally with both antigens. Both antibodies had a higher reactivities with HD antigen(s) in sera of five of eight lung cancer patients than 54 normal human sera. Since four of the above five sera had no abnormal titers to GP, it was concluded that their immunological status was antigen excess. The chicken antibody may be useful in follow-up studies of cancer patients to correlate the expression of HD antigen in tissues and sera with the elevation of HD antibodies, offering alternative methods of clinical prognosis of tumor growth and/or metastases. The human HD antibody may also be useful for the detection of HD antigens of glycoprotein nature.
通过放射免疫分析（RIA）比较了人源汉努齐乌-戴歇尔（HD）抗体和鸡抗N-糖基神经氨酰乳糖神经酰胺（HD3）抗体与HD抗原活性神经节苷脂（HD3）和一种糖蛋白（GP）的反应。人源抗体与糖蛋白的反应性高50倍，而鸡抗体与两种抗原的反应相同。与54份正常人血清相比，两种抗体在8例肺癌患者中的5例血清中与HD抗原的反应性更高。由于上述5份血清中的4份对GP没有异常滴度，因此得出结论，它们的免疫状态为抗原过量。鸡抗体可能有助于癌症患者的随访研究，以将组织和血清中HD抗原的表达与HD抗体的升高相关联，提供肿瘤生长和/或转移临床预后的替代方法。人源HD抗体也可能有助于检测糖蛋白性质的HD抗原。
