Mukuria J C, Naiki M, Hashimoto M, Nishiura K, Okabe M, Kato S
J Immunol Methods. 1985 Jun 12;80(1):97-106. doi: 10.1016/0022-1759(85)90168-1.
A relatively simple, specific and sensitive radioimmunoassay system has been developed for the detection of heterophile Hanganutziu-Deicher (H-D) antigen(s) and antibodies. The 125I-labeled H-D antigen-active molecule used for the assay is a bovine erythrocyte major glycoprotein previously found to have a strong H-D antigen potency. The antigen-antibody complex was precipitated with normal human serum as the carrier protein, followed by the addition of rabbit anti-human IgG F(ab')2 serum. With this method, different H-D antigen-active molecules were compared for heterophile H-D antigen potency with reasonable sensitivity detecting about 0.3 ng of cold glycoprotein. 8 different lung cancer tissues were assayed for H-D antigen. The sera from the 8 lung cancer patients were also screened by ELISA and RIA in an attempt to correlate expression of H-D antigen on tissues with elevation of H-D antibodies. The results showed that all patients' tissues expressed the antigen(s) but only 3 of them had abnormal levels of H-D antibodies. This could have been due to excess antigens in circulation or immune complexes.
已开发出一种相对简单、特异且灵敏的放射免疫分析系统，用于检测嗜异性汉-德（H-D）抗原和抗体。用于该分析的125I标记的具有H-D抗原活性的分子是一种牛红细胞主要糖蛋白，此前发现其具有很强的H-D抗原效力。抗原-抗体复合物用正常人血清作为载体蛋白沉淀，随后加入兔抗人IgG F(ab')2血清。用这种方法，比较了不同具有H-D抗原活性的分子的嗜异性H-D抗原效力，其灵敏度合理，能检测到约0.3 ng的冷糖蛋白。对8种不同的肺癌组织进行了H-D抗原检测。还通过酶联免疫吸附测定（ELISA）和放射免疫分析（RIA）对8例肺癌患者的血清进行了筛查，试图将组织中H-D抗原的表达与H-D抗体的升高联系起来。结果显示，所有患者的组织均表达了该抗原，但其中只有3例患者的H-D抗体水平异常。这可能是由于循环中抗原过量或免疫复合物所致。
