酵母核糖体核糖核酸的研究。III. 18S和26S酵母核糖体RNA及其复合物的二级结构：圆二色性和红外分析。

Studies on ribosomal ribonucleic acid from yeast. III. Secondary structure of 18 and 26S yeast ribosomal RNA's and their complex: circular dichroism and infrared analyses.

作者信息

Yanagi K, Katsura T, Iso K

出版信息

J Biochem. 1975 Sep;78(3):599-604. doi: 10.1093/oxfordjournals.jbchem.a130945.

DOI:10.1093/oxfordjournals.jbchem.a130945

Abstract

Circular dichroism (CD) and infrared (IR) spectroscopic analyses were performed of 26 and 18S yeast ribosomal RNA's (rRNA) and their specific complex, 30S RNA. The molecular ellipticity coefficients [thota] of 18, 26, and 30S rRNA's were 2.72, 2.63, and 2.65X10(4) degree-cm2/decimole at 264 nm, respectively. The base-pairing contents of 18, 26, and 30S rna's determined by iC), and 70% (32% AU, 38% GC), respectively. These results suggest that 18 and 26S rRNA have very similar secondary structures, and that 30S rna may have a slightly higher base-pairing content than the estimated sum of those of 18 and 26S rRNA's. The biological significance of this phenomenon is discussed in this report.

摘要

对26S和18S酵母核糖体RNA（rRNA）及其特定复合物30S RNA进行了圆二色性（CD）和红外（IR）光谱分析。18S、26S和30S rRNA在264nm处的分子椭圆率系数[θ]分别为2.72、2.63和2.65×10⁴度·厘米²/十分摩尔。通过红外光谱（IR）测定的18S、26S和30S rRNA的碱基配对含量分别为68%（36% AU，32% GC）、65%（33% AU，32% GC）和70%（32% AU，38% GC）。这些结果表明，18S和26S rRNA具有非常相似的二级结构，并且30S rRNA的碱基配对含量可能略高于18S和26S rRNA估计含量之和。本报告讨论了这一现象的生物学意义。