[使用DNA-DNA杂交方法研究白喉棒状杆菌菌株]
[Use of a DNA-DNA hybridization method for studying Corynebacterium diphtheriae strains].
作者信息
Mikhaĭlovich V M, Zaikin V L, Mazurova I K
出版信息
Mol Gen Mikrobiol Virusol. 1994 Nov-Dec(6):27-9.
PMID:7739593
Abstract
In addition to traditional tests, a battery of DNA probes was used to characterize 134 toxigenic and 125 nontoxigenic C.diphtheriae strains isolated in Russia in 1986-1989. 2.5% of nontoxigenic strains carried the determinants of both toxin subunits A and B, and 20% possessed at least a fragment of the gene determining toxin synthesis. Optimal conditions of hybridization technology were found. The method may be used as an alternative to traditional techniques in diagnostic studies and screenings.
摘要
除了传统检测方法外，还使用了一系列DNA探针来鉴定1986年至1989年在俄罗斯分离出的134株产毒白喉杆菌菌株和125株无毒白喉杆菌菌株。2.5%的无毒菌株携带毒素A和B两个亚基的决定簇，20%的菌株至少拥有决定毒素合成的基因片段。发现了杂交技术的最佳条件。该方法可作为诊断研究和筛查中传统技术的替代方法。
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