Uijthof J M, de Hoog G S, de Cock A W, Takeo K, Nishimura K
Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.
Mycoses. 1994 Jul-Aug;37(7-8):235-42. doi: 10.1111/j.1439-0507.1994.tb00419.x.
Strains of Exophiala dermatitidis, mainly originating from patients with systemic neurotropic mycosis in Asia and from the lungs of patients with cystic fibrosis (CF) in Europe, were analysed by ribotyping of the small subunit rDNA and by random amplification of polymorphic DNA (RAPD). A characteristic banding pattern for the species was found after restriction analysis of amplified fragments V9 and ITS4. The small subunit rDNA gene of five strains was about 1800 base pairs (bp) long, while in 16 strains its length was about 3000 bp. Using RAPD, seven populations could be distinguished. European CF strains as well as Asian strains from systemic mycoses are mainly distributed over two populations, one of which contained both CF strains and a systemic strain. It is concluded that the two clinical pictures are caused by genetically similar strains. The differences in pathogenicity may be explained by immunological differences in the hosts or by differences in exposure to the fungal propagules.
对主要来源于亚洲系统性嗜神经真菌病患者以及欧洲囊性纤维化（CF）患者肺部的皮炎外瓶霉菌株，通过小亚基rDNA的核糖体分型和随机扩增多态性DNA（RAPD）进行分析。对扩增片段V9和ITS4进行限制性分析后，发现了该菌种的特征性条带模式。5个菌株的小亚基rDNA基因长度约为1800个碱基对（bp），而16个菌株的长度约为3000 bp。使用RAPD可区分出7个群体。欧洲CF菌株以及来自系统性真菌病的亚洲菌株主要分布在两个群体中，其中一个群体同时包含CF菌株和一个系统性菌株。得出的结论是，这两种临床表现是由基因相似的菌株引起的。致病性差异可能是由宿主的免疫差异或接触真菌繁殖体的差异来解释。
