Phenotypic changes induced by replating of early post-natal rat chromaffin cells.

Postnatal chromaffin cells from rat adrenal medulla in culture respond to NGF by expressing neuronal traits. The replating of chromaffin cells after trypsinization produced neurite growth in a manner similar to that of NGF. The combination of replating and NGF exposure did not induce phenotypic changes over and above those observed by NGF alone. The morphological changes are independent of the preliminary culture conditions. The results of this study demonstrate for the first time that simple replating of young chromaffin cells can induce neuronal traits indistinguishable from those observed with NGF.