Lee J H, Klein H G
Department of Transfusion Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland, USA.
Transfusion. 1995 May;35(5):384-8. doi: 10.1046/j.1537-2995.1995.35595259147.x.
Granulocyte collection relies on the use of a red cell-sedimenting agent and is influenced by poorly defined intrinsic donor characteristics. The donor's baseline red cell (erythrocyte) sedimentation rate (ESR), a readily measurable intrinsic donor variable, may influence the effectiveness of red cell-sedimenting agents and affect the granulocyte yield.
The in vitro and in vivo effects of 6-percent hydroxyethyl starch on the donor ESR were prospectively studied in 67 granulocytapheresis procedures with a blood cell separator, and the findings were correlated with granulocyte collection efficiency (GCE). A relationship that predicts the GCE on the basis of the donor ESR was then derived and tested.
The 3.7-fold mean increase in the donor ESR measured after in vitro addition of hydroxyethyl starch approximated the 3.3-fold increase measured when it was administered to the donors. Higher baseline ESR correlated with larger in vitro and in vivo hydroxyethyl starch-induced increases in ESR and predicted more efficient cell collections and greater cell yields. Furthermore, both ESR and GCE, which varied significantly among donors, remained constant for a given donor undergoing repeat procedures. The results could be summarized by a simple predictive formula that relates the GCE (%) to the ESR (mm/hour): GCE = 1.3ESR + 45. The GCE and yield as predicted by the formula were accurate within 10 percent of the observed values in six subsequent procedures.
In granulocyte harvests using a blood cell separator with hydroxyethyl starch as the sedimenting agent, 1) both ESR and GCE vary widely among donors yet may remain relatively constant for a given donor; 2) the baseline ESR correlates with hydroxyethyl starch-modified ESR and with GCE; and 3) it may be possible to predict GCE and yield from the donor's baseline ESR.
粒细胞采集依赖于红细胞沉降剂的使用，并且受到尚不明确的内在供者特征的影响。供者的基线红细胞沉降率（ESR）是一个易于测量的内在供者变量，可能会影响红细胞沉降剂的效果并影响粒细胞产量。
前瞻性地研究了6%羟乙基淀粉对供者ESR的体外和体内效应，共进行了67例使用血细胞分离机的粒细胞单采程序，并将结果与粒细胞采集效率（GCE）相关联。然后推导并测试了基于供者ESR预测GCE的关系。
体外添加羟乙基淀粉后测得的供者ESR平均增加3.7倍，与给供者使用时测得的3.3倍增加相近。较高的基线ESR与体外和体内羟乙基淀粉诱导的ESR更大增加相关，并预测细胞采集更有效且细胞产量更高。此外，ESR和GCE在供者之间差异显著，但对于接受重复程序的给定供者保持不变。结果可以用一个简单的预测公式总结，该公式将GCE（%）与ESR（mm/小时）相关联：GCE = 1.3ESR + 45。该公式预测的GCE和产量在随后的6个程序中与观察值的误差在10%以内。
在使用羟乙基淀粉作为沉降剂的血细胞分离机进行粒细胞采集时，1）ESR和GCE在供者之间差异很大，但对于给定供者可能保持相对恒定；2）基线ESR与羟乙基淀粉修饰的ESR以及GCE相关；3）有可能从供者的基线ESR预测GCE和产量。
