Szymanski I O
Vox Sang. 1983;44(2):106-14. doi: 10.1111/j.1423-0410.1983.tb04111.x.
A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average efficiency of granulocyte collection by this method is about 64%. The average yield of WBC from 2,600 ml of donors' whole blood is 1.64 +/- 0.54 x 10(10) (mean +/- 1 SD) and the average granulocyte yield is 1.33 +/- 0.51 x 10(10) (mean +/- 1 SD). The granulocytes were shown to migrate to the sites of infection in vivo. This approach is an improvement over standard discontinuous flow leukapheresis because of the high efficiency of granulocyte collection and because multiple procedures are possible without exposing donors to more HES than is currently given during a single procedure.
本文介绍了一种采用间断流动离心法和低剂量羟乙基淀粉（HES）的新型粒细胞采集方法。按照该程序，血清多糖浓度的增加为100mg/dl，约为标准间断流动白细胞单采术后所见增加量的20%。通过该方法采集粒细胞的平均效率约为64%。从2600ml供体全血中获得的白细胞平均产量为1.64±0.54×10¹⁰（均值±1标准差），粒细胞平均产量为1.33±0.51×10¹⁰（均值±1标准差）。体内实验表明，采集的粒细胞能够迁移至感染部位。这种方法相较于标准间断流动白细胞单采术有所改进，原因在于粒细胞采集效率高，且可以进行多次操作，而不会使供体接触比目前单次操作更多的HES。
