Prieto C, García F J, Suárez P, Imáz M, Castro J M
Universidad Complutense, Facultad de Veterinaria, Departamento de Patologia Animal I, Madrid, Spain.
Zentralbl Veterinarmed B. 1994 Nov;41(9):608-17. doi: 10.1111/j.1439-0450.1994.tb00271.x.
A total of 522 Streptococcus suis isolates were recovered from the tonsils of slaughtered pigs by culturing on media containing different specific antisera. The serogroup reactions were determined by coagglutination. The most frequently isolated serotype was serotype 4 (representing 28.7% of the isolates), followed by serotype 3 (24.9%) and serotype 2 (22.6%). Typable isolates were examined by growth in 6.5% NaCl, amylase activity, acetoin production, susceptibility to optochin and production of acid from mannitol, melibiose, raffinose, salicin, sorbitol and trehalose. Finally, all isolates were tested for antimicrobial susceptibility against ampicillin, cephalotin, lincomycin, neomycin, penicillin, spiramycin and trimethoprim-sulphamethoxazole using the disk-diffusion technique. All isolates were amylase-positive and acetoin-negative, and failed to grow in 6.5% NaCl broth. Nine isolates were optochin-susceptible. Moreover, 10 isolates produced acid from mannitol, 21 produced acid from sorbitol, and 36 and 37 did not produce acid from salicin and trehalose, respectively. Six salicin- and trehalose-negative isolates and two mannitol- and sorbitol-fermenting isolates were found. Biochemical differences between serotypes were not observed. The most active drugs against S. suis were the beta-lactam antibiotics, although only 83.3%, 70% and 78.5% of isolates were susceptible to ampicillin, cephalotin and penicillin, respectively. Half of the isolates were resistant to trimethoprim-sulphamethoxazole, and resistance to lincomycin, neomycin and spiramycin appeared to be very high.
通过在含有不同特异性抗血清的培养基上培养，从屠宰猪的扁桃体中总共分离出522株猪链球菌。通过协同凝集测定血清群反应。最常分离出的血清型是4型（占分离株的28.7%），其次是3型（24.9%）和2型（22.6%）。对可分型的分离株进行了在6.5%氯化钠中生长、淀粉酶活性、乙酰甲基甲醇产生、对奥普托欣的敏感性以及从甘露醇、蜜二糖、棉子糖、水杨苷、山梨醇和海藻糖产酸的检测。最后，使用纸片扩散法对所有分离株进行了对氨苄西林、头孢噻吩、林可霉素、新霉素、青霉素、螺旋霉素和甲氧苄啶 - 磺胺甲恶唑的药敏试验。所有分离株淀粉酶阳性且乙酰甲基甲醇阴性，在6.5%氯化钠肉汤中不能生长。9株分离株对奥普托欣敏感。此外，10株分离株从甘露醇产酸，21株从山梨醇产酸，36株和37株分别不能从水杨苷和海藻糖产酸。发现6株水杨苷和海藻糖阴性分离株以及2株甘露醇和山梨醇发酵分离株。未观察到血清型之间的生化差异。对猪链球菌最有效的药物是β - 内酰胺类抗生素，尽管分别只有83.
