Chadee D D, Beier J C
Insect Vector Control Division, Ministry of Health, St. Joseph, Trinidad.
Ann Trop Med Parasitol. 1995 Feb;89(1):55-62. doi: 10.1080/00034983.1995.11812929.
The blood-feeding kinetics of Anopheles albitarsis, An. aquasalis, An. bellator and An. homunculus were determined under laboratory conditions using females collected from two sites in Trinidad. Many An. aquasalis, An. albitarsis and An. bellator completed probing within 30 s but An. homunculus averaged > 50 s. Anopheles albitarsis (68.3 s), An. aquasalis (67.0 s) and An. homunculus (77.7 s) took similar times to fill their guts but An. bellator took longer (106.5 s). More than 75% of the An. albitarsis, An. aquasalis and An. homunculus but only 51% of the An. bellator showed apparent prediuresis. Anopheles aquasalis exhibited the shortest mean duration of prediuresis (77.3 s) and An. homunculus the longest (152.9 s). Most individual mosquitoes spent less time filling their guts than in prediuresis. Overall, An. aquasalis exhibited the shortest total feeding time (135.7 s) and An. homunculus the longest (197.0 s). There was a considerable degree of variation among individuals of each species in their ability to agglutinate human erythrocytes immediately upon blood engorgement. For example, within the same feeding experiments, some individuals agglutinated < 25% of the ingested erythrocytes whereas others agglutinated > or = 90%. The parity of the field-collected anophelines had no significant effect on probing, duration of blood-feeding, duration of prediuresis, or erythrocyte agglutination. The inter- and intra-specific variations in blood-feeding and erythrocyte processing observed for the four species of field-collected anophelines from Trinidad are discussed in terms of vector competence and the evolution of these behaviours.
在实验室条件下，使用从特立尼达两个地点采集的雌性按蚊，测定了白跗按蚊、环喙按蚊、战斗按蚊和微小按蚊的吸血动力学。许多环喙按蚊、白跗按蚊和战斗按蚊在30秒内完成刺探，但微小按蚊平均超过50秒。白跗按蚊（68.3秒）、环喙按蚊（67.0秒）和微小按蚊（77.7秒）填满肠道的时间相似，但战斗按蚊花费的时间更长（106.5秒）。超过75%的白跗按蚊、环喙按蚊和微小按蚊出现明显的排尿前期，但战斗按蚊只有51%。环喙按蚊排尿前期的平均持续时间最短（77.3秒），微小按蚊最长（152.9秒）。大多数个体蚊子填满肠道的时间比排尿前期短。总体而言，环喙按蚊的总摄食时间最短（135.7秒），微小按蚊最长（197.0秒）。每个物种的个体在饱血后立即凝集人类红细胞的能力上存在相当大的差异。例如，在相同的摄食实验中，一些个体凝集的摄入红细胞不到25%，而另一些个体凝集的红细胞大于或等于90%。野外采集的按蚊的孕卵情况对刺探、吸血持续时间、排尿前期持续时间或红细胞凝集没有显著影响。根据媒介能力和这些行为的进化，讨论了从特立尼达采集的四种野外按蚊在吸血和红细胞处理方面的种间和种内差异。
