Rutherford R B, Spångberg L, Tucker M, Rueger D, Charette M
University of Michigan School of Dentistry, Ann Arbor 48109, USA.
Arch Oral Biol. 1994 Oct;39(10):833-8. doi: 10.1016/0003-9969(94)90014-0.
Recombinant human osteogenic protein-1 (hOP-1, BMP-7) induces cartilage and bone formation when implanted in extra- and intraskeletal sites in vivo. rOP-1 also preserves pulp vitality and stimulates reparative dentine formation when placed on partially amputated vital dental pulp tissue. The amount of dentine formed in 6 weeks was earlier found to be proportional to the total amount of rOP-1/carrier placed on the pulp and the capacity of the pulp to respond to rOP-1 appeared to be independent of the amount of coronal pulp removed. This reparative dentine was not completely mineralized after 6 weeks healing. Experiments were now made to determine the capacity of hOP-1 to preserve the vitality of and induce reparative dentine in vital radicular pulps. The extent of tissue mineralization present after 1, 2, 4 and 6 months' healing time in permanent monkey teeth was assessed. Radicular pulp vitality was maintained, reparative dentine formed, and mineralization was nearly 75% complete after 1 month and more than 95% after 4 months. The effects of irrigating the exposed pulps with EDTA, sodium hypochlorite or saline were also compared. Significantly more sodium hypochlorite-treated pulps became non-vital and the root canals of all the non-vital teeth contained bacteria at the time of sacrifice.
重组人骨生成蛋白-1（hOP-1，骨形态发生蛋白-7）植入体内骨骼外和骨骼内部位时可诱导软骨和骨形成。将rOP-1置于部分切断的活牙髓组织上时，它还能保持牙髓活力并刺激修复性牙本质形成。早期发现，6周内形成的牙本质量与置于牙髓上的rOP-1/载体总量成正比，牙髓对rOP-1的反应能力似乎与切除的冠髓量无关。愈合6周后，这种修复性牙本质并未完全矿化。现在进行实验以确定hOP-1保持活根髓活力并诱导其形成修复性牙本质的能力。评估了恒河猴牙齿在愈合1、2、4和6个月后组织矿化的程度。根髓活力得以维持，形成了修复性牙本质，1个月后矿化接近75%，4个月后超过95%。还比较了用乙二胺四乙酸（EDTA）、次氯酸钠或生理盐水冲洗暴露牙髓的效果。用次氯酸钠处理的牙髓明显更多地失去活力，在处死时所有失活牙齿的根管中都含有细菌。
