Nakashima M
Department of Conservative Dentistry, Faculty of Dentistry, Kyushu University Fukuoka, Japan.
J Dent Res. 1994 Sep;73(9):1515-22. doi: 10.1177/00220345940730090601.
Dental pulp cells have the potential to differentiate into odontoblasts. The molecular mechanisms underlying differentiation are not clear. Demineralized dentin matrix is osteoinductive and contains bone morphogenetic protein (BMP) activity. BMPs have been implicated in embryonic odontogenic differentiation and hence may play a role in the differentiation of adult pulp cells into odontoblasts during pulpal healing. This study examined the hypothesis that BMPs induce dentin formation on amputated canine pulp. Recombinant human BMP-2 and BMP-4 were capped with inactivated dentin matrix on amputated pulp. At two months, the amputated pulp was filled with tubular dentin in the lower part and osteodentin in the upper part. The amount of dentin formed was markedly diminished when dentin matrix alone was implanted. These findings imply that recombinant human BMP-2 and BMP-4 induce differentiation of adult pulp cells into odontoblasts. Thus, BMPs may have a role in dentistry as a bioactive pulp-capping agent to induce dentin formation.
牙髓细胞具有分化为成牙本质细胞的潜力。其分化的分子机制尚不清楚。脱矿牙本质基质具有骨诱导性，并含有骨形态发生蛋白（BMP）活性。BMPs与胚胎期牙源性分化有关，因此在牙髓愈合过程中，可能在成体牙髓细胞向成牙本质细胞的分化中发挥作用。本研究检验了BMPs诱导犬牙髓切断处形成牙本质这一假说。重组人BMP - 2和BMP - 4与失活的牙本质基质一起覆盖于切断的牙髓上。两个月时，切断的牙髓下部充满管状牙本质，上部充满骨样牙本质。单独植入牙本质基质时，形成的牙本质量明显减少。这些发现表明，重组人BMP - 2和BMP - 4可诱导成体牙髓细胞分化为成牙本质细胞。因此，BMPs作为一种生物活性盖髓剂诱导牙本质形成，在牙科领域可能具有重要作用。
