Nikawa H, Samaranayake L P, Tenovuo J, Hamada T
Oral Biology Unit, Prince Phillip Dental Hospital, University of Hong Kong.
Arch Oral Biol. 1994 Oct;39(10):921-3. doi: 10.1016/0003-9969(94)90026-4.
The effect of subminimal inhibitory concentrations (MICs) of nystatin, amphotericin B, clotrimazole, miconazole and 5-fluorocytosine on the in vitro susceptibility of Candida albicans to physiological concentrations of apo-lactoferrin was investigated. Pre-exposure of C. albicans in 1:4 and 1:16 MICs of the antifungals resulted in an increased resistance to apo-lactoferrin-mediated cell death. Preincubation of Candida in tunicamycin (an agent that inhibits the synthesis of yeast cell-wall mannoproteins) and subsequent exposure to apo-lactoferrin enhanced the antifungal activity, whilst addition of ergosterol (a yeast cell-wall component) to the assay suspension had no significant effect. These results taken together indicate that apo-lactoferrin, an important component of saliva, interacts with cell-membrane constituents of C. albicans such as mannoproteins, and may modulate the effect of topical antifungal agents commonly prescribed for oral candidoses.
研究了制霉菌素、两性霉素B、克霉唑、咪康唑和5-氟胞嘧啶的亚最小抑菌浓度（MIC）对白色念珠菌体外对生理浓度脱铁乳铁蛋白敏感性的影响。白色念珠菌在抗真菌药物的1:4和1:16 MIC中预暴露导致对脱铁乳铁蛋白介导的细胞死亡的抗性增加。念珠菌在衣霉素（一种抑制酵母细胞壁甘露糖蛋白合成的药物）中预孵育，随后暴露于脱铁乳铁蛋白可增强抗真菌活性，而向测定悬浮液中添加麦角固醇（一种酵母细胞壁成分）则无显著影响。综合这些结果表明，脱铁乳铁蛋白作为唾液的一种重要成分，可与白色念珠菌的细胞膜成分如甘露糖蛋白相互作用，并可能调节常用于治疗口腔念珠菌病的局部抗真菌药物的效果。
