Renedo D E, Trainer T D
Department of Pathology, University of Vermont, Burlington 05405.
Am J Surg Pathol. 1994 Sep;18(9):947-52. doi: 10.1097/00000478-199409000-00010.
Intratubular germ cell neoplasia (ITGCN) and mature teratoma of the testis are uncommon findings in children. We report a case of a 3-year-old boy with both ITGCN and mature teratoma--a unique finding in our experience. Immunohistochemical markers, including placental alkaline phosphatase (PLAP), 43-9F, p53, and proliferating cell nuclear antigen (PCNA), as well as the periodic acid-Schiff (PAS) stain, were applied to the ITGCN. PLAP and 43-9F were not detected, whereas p53 and PCNA nuclear expression was detected in approximately 5% of atypical germ cells. Abundant clumped intracytoplasmic glycogen deposits were identified within atypical germ cells. Our findings indicate that both PCNA and p53, in addition to a PAS stain, may be useful markers in detecting malignant intratubular germ cells.
睾丸内生殖细胞瘤（ITGCN）和睾丸成熟畸胎瘤在儿童中并不常见。我们报告一例3岁男孩同时患有ITGCN和成熟畸胎瘤的病例——据我们经验，这是一个独特的发现。免疫组织化学标志物，包括胎盘碱性磷酸酶（PLAP）、43-9F、p53和增殖细胞核抗原（PCNA），以及过碘酸-希夫（PAS）染色，被应用于ITGCN。未检测到PLAP和43-9F，而在约5%的非典型生殖细胞中检测到p53和PCNA核表达。在非典型生殖细胞内发现大量聚集的胞浆内糖原沉积。我们的研究结果表明，除PAS染色外，PCNA和p53可能都是检测睾丸内恶性生殖细胞的有用标志物。
