Hawkins E, Heifetz S A, Giller R, Cushing B
Department of Pathology, Baylor College of Medicine, Houston, TX, USA.
Hum Pathol. 1997 Apr;28(4):404-10. doi: 10.1016/s0046-8177(97)90027-7.
Seminiferous tubules adjacent to germ cell tumors (GCT) in prepubertal boys frequently contain increased germ cells with abundant, clear cytoplasm. These cells are placental alkaline phosphatase (PLAP) negative and are usually not considered to represent intratubular germ cell neoplasia (ITGCN). A recent case report found p53 and proliferating cell nuclear antigen (PCNA) positivity in such cells and equated these PLAP-negative cells with ITGCN. Because the proto-oncogene c-kit is also a marker of ITGCN, immunohistochemical tests for c-kit and PLAP were performed on 28 testes adjacent to prepubertal GCT in children aged 2 to 45 months. Additional slides from testes not associated with GCT from 18 preterm infants and children ages 19 weeks to 7 years were also tested. An adult testis with seminoma and ITGCN served as a positive control. PCNA, PLAP, and p53 were tested on available slides. No intratubular germ cells adjacent to GCT in prepubertal children were positive for PLAP or c-kit; five of seven were positive for PCNA; p53 was present in the two examined. These results indicate that germ cells adjacent to infantile GCT are proliferative but not neoplastic and offer additional evidence that intratubular germ cells and GCT in prepubertal boys are different from those of adolescents and adults.
青春期前男孩中，与生殖细胞肿瘤（GCT）相邻的生精小管常常含有增多的生殖细胞，这些细胞胞质丰富且清亮。这些细胞胎盘碱性磷酸酶（PLAP）阴性，通常不被认为代表小管内生殖细胞肿瘤（ITGCN）。最近一份病例报告发现这些细胞中p53和增殖细胞核抗原（PCNA）呈阳性，并将这些PLAP阴性细胞等同于ITGCN。由于原癌基因c-kit也是ITGCN的一个标志物，因此对2至45个月大儿童青春期前GCT相邻的28个睾丸进行了c-kit和PLAP免疫组化检测。还检测了18例19周龄至7岁早产儿和儿童与GCT不相关的睾丸的额外切片。一个患有精原细胞瘤和ITGCN的成人睾丸用作阳性对照。对可用切片检测了PCNA、PLAP和p53。青春期前儿童GCT相邻的小管内生殖细胞PLAP或c-kit均为阴性；7例中有5例PCNA阳性；检测的2例中有p53表达。这些结果表明，婴儿GCT相邻的生殖细胞具有增殖性但并非肿瘤性，并且进一步证明青春期前男孩的小管内生殖细胞和GCT与青少年及成人的不同。
